How Synthetic RNA makes CRISPR Better

CRISPR is a precise molecular tool for gene editing research. Currently, CRISPR experiments are dominated by plasmid-based approaches which trade off precision and accuracy for cost. Precision and accuracy are lost from the constant overexpression of Cas9 protein and the guide RNA from plasmids. Plasmids are also susceptible to differential expression leading to reduced precision, accuracy and subsequent reproducibility.

A more reliable approach is to use synthetic RNA instead of plasmid DNA. Synthetic RNA can minimize off-target activity by existing for only a defined amount of time before it naturally degrades.

Increasingly, CRISPR research employs high throughput methods like phenotypic screening, requiring hundreds to thousands of targets. However, current synthetic RNA options are unsuitable, being either too expensive or low throughput.

A fast, affordable and scalable synthetic RNA synthesis platform is needed, and Spindle Biotech is here to help.

Spindle Biotech offers a unique solution by providing synthetic RNA at a low cost and in high throughput. This provides small and intermediate-sized laboratories the tools to perform higher throughput CRISPR research, enabling access to RNA-based experimentation on your own terms at previously unattainable throughput.